Fig. 5: dCas9-based transcriptional activation complexes targeted to the Nrf2 promoter induce α-synuclein clearance. | Cell Death & Disease

Fig. 5: dCas9-based transcriptional activation complexes targeted to the Nrf2 promoter induce α-synuclein clearance.

From: Targeted de-repression of neuronal Nrf2 inhibits α-synuclein accumulation

Fig. 5

A Neurons were transfected at DiV 8 with dCas9-VP64, p65 and sgRNAs targeting the Nrf2 promoter (controls were empty sgRNA vectors) with Nrf2-promoter Firefly luciferase and TK-Renilla control. Luciferase activity was measured at 96 h. *P = 0.032, Student’s t test (n = 4). B Nrf2+/+ and Nrf2−/− cortical neurons were transfected as in (A) in addition to α-synuclein expression vector and eGFP transfection marker. At Div 11 cells were fixed and processed for GFP and α-synuclein immunofluorescence as for Fig. 1. *P = 0.001, 2-Way ANOVA + Bonferroni’s multiple comparison test, 192–573 cells analysed per condition (n = 7 WT and 5 Nrf2 KO). C Example pictures.

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