Fig. 1: NEDD4 binds and ubiquitinates the SARS-CoV-2 S protein.
From: Inhibition of HECT E3 ligases as potential therapy for COVID-19
A Immunoblotting (IB) of flag-immunoprecipates (IP) and whole cell lysis (WCL) derived from HEK293T cells that were transfected with HA-SARS-CoV-2 S and indicated NEDD4 family members. B A schematic diagram to show the PPxY motif in the SARS-CoV-2 S protein. C IB of flag-immunoprecipates and WCL derived from HEK293T cells that were transfected with NEDD4 and SARS-CoV-2 S WT or mutants. D IB of Ni-NTA pulldown and WCL derived from HEK293T cells that were transfected with SARS-CoV-2 S and NEDD4 WT (or EV as a negative control).
