Fig. 5: Gain-of-Function mutants in NEDD4, but not SMURF1 and WWP1, display elevated interaction with, but comparable ubiquitination of the SARS-CoV-2 S protein.
From: Inhibition of HECT E3 ligases as potential therapy for COVID-19
A, C IB of flag-immunoprecipates and WCL derived from HEK293T cells that were transfected with HA-SARS-CoV-2 S and NEDD4/WWP1 WT and mutants. B, D IB of Ni-NTA pulldown and WCL derived from HEK293T cells that were transfected with SARS-CoV-2 S and NEDD4/WWP1 WT and mutants. E IB of flag-immunoprecipates and WCL derived from HEK293T cells that were transfected with HA-SARS-CoV-2 S and WT and mutants for Smurf1 or WWP1. F IB of Ni-NTA pulldown and WCL derived from HEK293T cells that were transfected with SARS-CoV-2 S and WT and mutants for Smurf1 or WWP1. G Binding and ubiquitination activity in WWP1/NEDD4/SMURF1 mutants vs. wild-type (WT).
