Table 2 Real-Time PCR primer sequences.
From: Inhibition of HECT E3 ligases as potential therapy for COVID-19
Gene | Accession number | Sequence (5’→3’) | Product size (bp) | |
|---|---|---|---|---|
WWP1 | NM_007013.4 | Fw | TGTAAATGTTACGCCACAGACT | 105 |
Rv | GCTTGTTTCAAATCTATCGTTGC | |||
WWP2 | NM_007014.5 | Fw | GAAAGTGGTGTCCGCAAAGC | 175 |
Rv | ATGACTCTGTGCCGTGACATT | |||
NEDD4 | NM_006154.4 | Fw | CTGCTACGGACAATTATACCCTA | 129 |
Rv | CATCCAACAGTTTGCCATGATA | |||
NEDD4L | NM_001144967.3 | Fw | ACGTAGCGGATGAGAATAGAGAAC | 115 |
Rv | CTGTGATTAGATGGGTTTACCCTGA | |||
ITCH | NM_031483.7 | Fw | GGTTCAGTATTTCCGGTTCTGGT | 118 |
Rv | GGGACTGAAGCTCATTATCTGTTG | |||
SMURF1 | NM_020429.3 | Fw | CCGCTCCAAGGCTTCAAGG | 125 |
Rv | ATCCGGTTAAAGCAGGTATGGG | |||
SMURF2 | NM_022739.4 | Fw | GCAAATGGATCAGGAAGTCGGAAA | 100 |
Rv | CCGGAGGCCGGAGGA | |||
HECW1 | NM_015052.5 | Fw | CGAGCAACCACCCCCAGTGT | 136 |
Rv | CCATGGCTTGGAAATCTGAGAGA | |||
HECW2 | NM_001348768.2 | Fw | CTACCAGCATAACCGCGACC | 112 |
Rv | AAAGAATGCCTTGCCCTGGT | |||
GAPDH | NM_002046 | Fw | AAGGTCGGAGTCAACGGATTT | 100 |
Rv | TGAAGGGGTCATTGATGGCA | |||
ACTB | NM_001101 | Fw | ATTGCCGACAGGATGCAGAA | 150 |
Rv | GCTGATCCACATCTGCTGGAA | |||
RPLP0 | NM_001002 | Fw | ACCCAGCTCTGGAGAAACT | 198 |
Rv | AAAAGGAGGTCTTCTCGGG | |||
