Fig. 4: hUC-MSCs suppress the activation and pro-inflammatory cytokine secretion of hepatic T cells through CHI3L1 in Con A-induced hepatitis. | Cell Death & Disease

Fig. 4: hUC-MSCs suppress the activation and pro-inflammatory cytokine secretion of hepatic T cells through CHI3L1 in Con A-induced hepatitis.

From: Mesenchymal stem cells alleviate experimental immune-mediated liver injury via chitinase 3-like protein 1-mediated T cell suppression

Fig. 4

A Representative plots and quantification (B) of flow cytometry analysis for CD3+ T cells in liver MNCs 24 h after Con A administration in indicated groups. C–F Representative plots and quantification of flow cytometry analysis for CD25 (C–D) and CD69 (E–F) expression in CD3+ T cells in liver MNCs 24 h after Con A administration in indicated groups. G–J Representative plots and quantification of flow cytometry analysis for IFN-γ (G–H) and TNF-α (I–J) -producing CD3+ T cells in liver MNCs 24 h after Con A administration in indicated groups. K Serum concentrations of IL6, IFN-γ, and TNF-α 24 h after Con A administration in indicated groups were detected by CBA kit. Analyses in (G–J) were conducted with liver MNCs stimulated with PMA (50 ng/mL), ionomycin (500 ng/mL) and BFA (10 μg/mL) for 6 h after isolation. Data in (B), (D), (F), (H), (J), and (K) are shown as mean ± SD (n = 5 for each group) with indicated significance (*p < 0.05, **p < 0.01).

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