Fig. 6: Inflammatory changes in the skin and small intestine elicited by EC challenge were attenuated in the antibiotic-treated mice. | Cell Death & Disease

Fig. 6: Inflammatory changes in the skin and small intestine elicited by EC challenge were attenuated in the antibiotic-treated mice.

From: Small intestinal immune-environmental changes induced by oral tolerance inhibit experimental atopic dermatitis

Fig. 6

A Schedule of antibiotic treatments. B Hematoxylin and eosin staining of skin from the antibiotic-treated control (Abx-Con), antibiotic-treated and EC-challenged (Abx-EC), and antibiotic-treated, orally tolerized, and EC-challenged (Abx-OT-EC) mice. The images are representative of two independent experiments. The scale bar represents 200 μm. C Whole skin thickness (left), epidermal thickness (middle), and dermal thickness (right) of the indicated mice. The graphs show the mean ± SD values. *P < 0.05, **P < 0.01, ****P < 0.0001 (one-way ANOVA). D mRNA expression of Il1b, Il1rl1, Il17a, Il25, Il33, Il5, Il13, Flg, Lor, Mcpt1, Ccr3, Prg2, and Ccl11 in the skin of the indicated mice. All data are representative of two independent experiments. The graphs show the mean ± SD values. *P < 0.05, **P < 0.01 (Student’s t-test for Il1b, I1rl1, Il5, Prg2, and Ccl11; Mann–Whitney test for Mcpt1 and Ccr3). E mRNA expression of Il1b, Il6, Tnf, Il17a, Il25, Il33, Il13, Cldn4, Mcpt1, Ccr3, Prg2, Ccl11, and Ccl5 in the small intestine of the indicated mice. All data are representative of two independent experiments. The graphs show the mean ± SD values. *P < 0.05, ***P < 0.001 (Student’s t-test for Tnf, Ccr3, and Ccl5; Mann–Whitney test for Il1b, Il17a, and Mcpt1).

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