Fig. 3: CPX impairs mitochondrial function. | Cell Death & Disease

Fig. 3: CPX impairs mitochondrial function.

From: Ciclopirox and bortezomib synergistically inhibits glioblastoma multiforme growth via simultaneously enhancing JNK/p38 MAPK and NF-κB signaling

Fig. 3

A U251 and SF126 cells were treated with CPX (0, 20, 40, and 80 µM for U251 cells; 0, 10, 20, and 40 µM for SF126 cells) for 48 h and indicated subunits of mitochondrial enzymatic complexes were detected by immunoblotting. B Mitochondrial ROS levels in U251 and SF126 cells following CPX, NAC, or combined treatments, were measured using a MitoSOX Red mitochondrial superoxide indicator kit. Data represent mean ± SD (n = 3; ***p < 0.001). C Mitochondrial respiration profiles of U251 (upper) and SF126 (lower) cells treated with (20 μM) or without CPX combined with NAC (10 mM). The representative graph represents the mean oxygen consumption rate (OCR) ± SD of six replicates. D–F Basal respiration (D), maximal respiration (E) and ATP production (F) of U251 and SF126 cells treated with or without CPX (40 µM) combined with NAC (10 mM). Data represent mean ± SD (n = 3; *p < 0.05; **p < 0.01; ***p < 0.001).

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