Fig. 5: The combination of CPX and BTZ promotes the activation of NF-κB signaling to induce SASP. | Cell Death & Disease

Fig. 5: The combination of CPX and BTZ promotes the activation of NF-κB signaling to induce SASP.

From: Ciclopirox and bortezomib synergistically inhibits glioblastoma multiforme growth via simultaneously enhancing JNK/p38 MAPK and NF-κB signaling

Fig. 5

A Immunoblotting analysis of p-p65, p65, and IκBα in U251 and SF126 cells treated with vehicle (DMSO), CPX (20 μM), BTZ (24 nM), or a combination of both drugs for 24 h. B U251 and SF126 cells were treated with vehicle (DMSO), CPX (20 μM) and BTZ (24 nM) either alone or in combination for 24 h, and cytosolic (Cyto) or nuclear (Nu) fractions were isolated, followed by immunoblotting analysis with primary antibodies against p65, p84, and GAPDH. C Immunofluorescent analysis for p65 cellular localization of U251 and SF126 cells treated with vehicle (DMSO), CPX (20 μM), and BTZ (24 nM), either alone or in combination. D mRNA levels of the SASP markers, IL6 and IL8, in U251 and SF126 cells treated with vehicle (DMSO), CPX (20 μM), and BTZ (24 nM), either alone or in combination.

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