Fig. 4: Validation of LXRα as a direct target of miR-155-5p. | Cell Death & Disease

Fig. 4: Validation of LXRα as a direct target of miR-155-5p.

From: CTRP12 ameliorates atherosclerosis by promoting cholesterol efflux and inhibiting inflammatory response via the miR-155-5p/LXRα pathway

Fig. 4

A Schematic of miR-155-5p binding site in the 3′ UTR of LXRα mRNA and corresponding mutation. B Calculation of free energy score by the RNAhybrid database. C The 293T cells were co-transfected with the luciferase reporter plasmids (LXRα-WT and LXRα-Mut) and miR-155-5p mimic or its negative control for 48 h. The luciferase activity was then determined. D, E After 48 h of treatment with ox-LDL at 50 µg/mL, THP-1 macrophages were transfected with miR-155-5p mimic/inhibitor or their negative controls for 48 h. D Analysis of miR-155-5p expression by qRT-PCR. E Detection of LXRα expression by both qRT-PCR and western blot. Data are represented as the mean ± SD from three independent experiments. ****P < 0.0001; ns not significant.

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