Fig. 6: Effects of CTRP12 on macrophage polarization and inflammatory cytokine expression. | Cell Death & Disease

Fig. 6: Effects of CTRP12 on macrophage polarization and inflammatory cytokine expression.

From: CTRP12 ameliorates atherosclerosis by promoting cholesterol efflux and inhibiting inflammatory response via the miR-155-5p/LXRα pathway

Fig. 6

A, B THP-1 macrophages were incubated with 50 µg/mL ox-LDL for 48 h, which was followed by transfection with PBS, LV-NC, or LV-CTRP12 for 72 h. The mRNA expression of iNOS, CD86, Mrc-1, Arg-1, MCP-1, TNF-α, and IL-10 was assayed using qRT-PCR. C–F THP-1 macrophages were loaded with 50 µg/mL ox-LDL for 48 h, transfected with LXRα siRNA or miR-155-5p mimic for another 48 h and then transduced with LV-CTRP12 for 72 h. The qRT-PCR was employed to measure the mRNA expression of iNOS, CD86, Mrc-1, Arg-1, MCP-1, TNF-α, and IL-10. Data are the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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