Fig. 2: miR-150 inhibits growth of OC cells and promotes apoptosis in vitro and in vivo.
From: FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop
A2780, SKOV3, and ES2 cells were transduced with mir-150-overexpressing lentivirus (Lv-mir-150) or control lentivirus (Lv-mir-NC). A, B Cell proliferation was determined by colony formation assay (A) and EdU incorporation assay (B). Scale bar: 100 µm. C Cell cycle was determined by flow cytometry. D Cell apoptotic rate was determined by flow cytometry of cells with Annexin V-PE/7AA-D double staining. E Levels of Cyclin D1, p27, p21, and p-Rb (Ser795) were detected in Lv-mir-150 cells by western blotting. F ES2 cells stably transfected with Lv-mir-150 (right) or Lv-mir-NC (left) were injected subcutaneously into female BALB/c nude mice (n = 6), and images of the tumors at autopsy from nude mice were presented (bottom). G Tumor volumes were measured at the indicated time points. H Average weight of xenografted tumors was measured. I Representative photographs of immunohistochemical (IHC) staining of Ki67 in xenografted tumors from Lv-mir-150-ES2 cells or control cells. Magnification: ×400. Scale bar: 50 µm. The data are presented as the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by Student’s t-test or two-way ANOVA.
