Fig. 3: miR-150 inhibits migratory and invasive ability of OC cells in vitro and in vivo.
From: FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop
A, B Stably overexpressing miR-150 reduced migration (A) and invasion (B) of OC cells in vitro. Scale bar: 200 µm. C Wound healing assay indicated that miR-150 overexpression inhibited healing ability of A2780 (top) and SKOV3 (bottom) cells. D–F Lv-mir-150 OC cells and corresponding Lv-mir-NC cells were intraperitoneally injected into female BALB/c nude mice that were euthanized after 45 days (for A2780 cells) or 24 days (for ES2 cells) (n = 5). D Images showing smaller ascites volumes (left) and abdominal circumference (right) in mice injected with Lv-mir-150 cells than those in control cells. E Fluorescence imaging revealed that mice injected with Lv-mir-150 cells had weaker green fluorescence signals on the mesentery. F Number of nodules was significantly lower in mice injected with Lv-mir-150 cells when compared with that of the control group. The data are presented as the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t-test.
