Fig. 3: Characterization of circCUX1 in HPSCC.

A The circCUX1 structure. B qRT-PCR products with divergent primers showing circularization of circCUX1. cDNA complementary DNA, gDNA genomic DNA. C qRT-PCR analysis for the expression of circCUX1 and CUX1 mRNA after treatment with RNase R in Fadu cells. D qRT-PCR analysis for the expression of circCUX1 and CUX1 mRNAs after treatment with Actinomycin D at the indicated time points in Fadu cells. E RNA fluorescence in situ hybridization for circCUX1. Nuclei were stained with DAPI.