Fig. 4: METTL3 promotes m6A-methylated circCUX1. | Cell Death & Disease

Fig. 4: METTL3 promotes m6A-methylated circCUX1.

From: N6-methyladenosine modification of circCUX1 confers radioresistance of hypopharyngeal squamous cell carcinoma through caspase1 pathway

Fig. 4

A RIP assay showing that circCUX1 was highly recruited in m6A precipitated fraction in Fadu cells. B RIP assays showing the association of METTL3 and METTL14 with circCUX1 in Fadu cells. Relative enrichment representing RNA levels associated with METTL3 and METTL14 relative to an input control. IgG antibody served as a control. C The circCUX1-protein complex pulled down by circCUX1 junction probe with protein extracts from Fadu cells. Immunoblot analysis of METTL3 after pull-down assay showing its specific association with circCUX1. D Western blot analysis for the expression of METTL3 after METTL3 siRNA transfection in Fadu cells. E qRT-PCR analysis for the expression of circCUX1 and CUX1 mRNA after METTL3 siRNA transfection in Fadu cells. F qRT-PCR analysis for the expression of circCUX1 after treatment with Actinomycin D at the indicated time points in Fadu cells.

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