Fig. 2: Rab26 Inhibits migration and invasion of breast cancer cells.

A Wound-healing assay revealed that over-expression of Rab26 inhibits migration of MDA-MB-231 cells. B Quantitative analysis of the wound area using imageJ software from three independent wound-healing experiments, **p < 0.01. C Transwell assays showed Rab26 over-expression inhibits migration of MDA-MB-231 cells. D Quantitative analysis of the results of C from three independent experiments, *p < 0.05. E Rab26 alters Golgi reorientation during migration in MDA-MB-231 cells. The Golgi apparatus was labeled with TGN46 antibody, bar = 20 μm. “+” and “−” means the Golgi facing in the direction of migration or not, respectively. Bar = 50 μm. F Quantitative analysis of the results of E from three independent experiments, **p < 0.01. G Matrigel assay showed Rab26 inhibits invasive ability of MDA-MB-231 cells. H Quantitative analysis of the results of G from three independent experiments, *p < 0.05. I Over-expression of Rab26 inhibits colony formation of MDA-MB-231 cells in soft agar. J Quantitative analysis of I from three independent experiments, **p < 0.01. K HE staining demonstrated that over-expression of Rab26 inhibits the metastasis of MDA-MB-231 cells and tumor formation in lung tissues of nude mice. Arrows indicates the tumors, bar = 3000 μm. L Quantitative analysis of K, n = 5. **p < 0.01.