Fig. 4: Rab26 downregulates the protein level of the active Src in breast cancer cells. | Cell Death & Disease

Fig. 4: Rab26 downregulates the protein level of the active Src in breast cancer cells.

From: Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src

Fig. 4

A MDA-MB-231 cells were co-transfected with GFP-Rab26 and pUSEamp-SrcCA; the results showed that Rab26 alters the intracellular localization of SrcCA under normal condition or EGF treatment. Bar = 20 μm. B Western blot demonstrated over-expression of Rab26 reduces phosphorylated Src in the presence of EGF (100 ng/ml) or not in MDA-MB-231 cells. C Quantitative analysis of the results of B from three independent experiments. **p < 0.01. D Western-blot demonstrated Rab26 knockdown increases the protein level of phosphorylated Src in MCF7 cells. E Quantitative analysis of the results of D from three independent experiments. *p < 0.05.

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