Fig. 5: Expression of the active Src reverses the Rab26-induced inhibition for cell migration and invasion.

A MDA-MB-231 cells stably expressing Rab26 were transfected with pUSEamp-SrcCA, western blot showing the expression of SrcCA. B Wound-healing assays showed SrcCA rescued migration of MDA-MB-231 cells expressing Rab26. C Quantitative analysis of the wound area using imageJ software from three independent wound-healing experiments, **p < 0.01, *p < 0.05. D, E Matrigel invasion assays showed SrcCA promotes invasion of MDA-MB-231 cells expressing Rab26. **p < 0.01. F Wound-healing assays showed inhibition of Src activity by PP2 inhibits migration of MCF7 cells with Rab26 depleted. G Quantitative analysis of the wound area using imageJ software from three independent wound-healing experiments, *p < 0.05.