Fig. 6: Rab26 induces the active Src targeting to autophagosome. | Cell Death & Disease

Fig. 6: Rab26 induces the active Src targeting to autophagosome.

From: Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src

Fig. 6

A GFP-Rab26 is associated with autophagosomes marked by LC3 antibody in MDA-MB-231 cells. Bar = 20 μm. B Chloroquine (CQ) treatment increases the association of Rab26 with LC3. Bar = 20 μm. C Quantitative analysis by ImageJ software to reveal Rab26/LC3-positive compartments. **p < 0.01. D Over-expression of Rab26 induces SrcCA targeting to LC3 marked autophagosomes. Bar = 20 μm. E Western blot showed over-expression of Rab26 increases the level of LC3II. F Quantitative analysis of the results of E from three independent experiments. *p < 0.05. G Western blot showed Rab26 knockdown decreases the level of LC3II. H Quantitative analysis of the results of G from three independent experiments. *p < 0.05.

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