Fig. 6: Acute PRRT2 deletion decreases the activation of NKA evoked by high-frequency stimulation.
From: An interaction between PRRT2 and Na+/K+ ATPase contributes to the control of neuronal excitability
A AHP induction protocol. Neurons were kept in whole-cell current-clamp configuration and APs were evoked by delivering supra-threshold current pulses at 50 Hz for 2 s. Inset: expanded view of the AHP phase indicated by the dashed box. The black dashed lines indicate the holding potential (−70 mV). B Representative AHP traces recorded from Scr (top) and PRRT2 KD (bottom) neurons before (ctrl) and after the application of ouabain (oua, 1 mM). The NKA-induced AHP was obtained by digital subtraction of the response in the presence of ouabain from the control response (green traces). Gray-shaded areas indicate the NKA-induced AHP component. C The areas under the AHP waveform, baselined to −70 mV and within a time window of 20 s after the train, were measured in Scr and PRRT2 KD neurons before and after ouabain blockade. D The areas of the NKA-dependent AHP in Scr and PRRT2 KD neurons were obtained from the difference between individual AHP areas before and after ouabain blockade (n = 19 and 20 for Scr and PRRT2 KD, respectively, from three independent neuronal preparations). Bar plots show the means ± SEM with superimposed individual experimental points. *p < 0.05, **p < 0.01, ***p < 0.001; two-way ANOVA/Šídák (C); unpaired Student’s t-test (D).
