Fig. 5: DKK1 decreased the expression of Wnt/β-catenin signaling in active cGVHD-MSCs. | Cell Death & Disease

Fig. 5: DKK1 decreased the expression of Wnt/β-catenin signaling in active cGVHD-MSCs.

From: Wnt/β-catenin signaling mediates the abnormal osteogenic and adipogenic capabilities of bone marrow mesenchymal stem cells from chronic graft-versus-host disease patients

Fig. 5

A Representative protein expression of Wnt3a, phosphor-GSK-3β, total-GSK-3β, phosphor-β-catenin, total-β-catenin, and β-tubulin in active cGVHD-MSCs treated or untreated with DKK1 (n = 12) and no cGVHD-MSCs (n = 12). B Relative protein expression of active cGVHD-MSCs treated or untreated with DKK1 (n = 12) and no cGVHD-MSCs (n = 12). C Relative gene expression of osteogenic/adipogenic marker gene. D Alizarin red staining, Orange-red represents calcium nodules. Oil Red O staining, red represents lipid droplets. No cGVHD-MSCs (n = 33), active cGVHD (n = 33) and DKK1 + cGVHD (n = 12). Black bars in each figure represent the median ± range. NS, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

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