Fig. 6: Immunomodulation of MSCs. | Cell Death & Disease

Fig. 6: Immunomodulation of MSCs.

From: Wnt/β-catenin signaling mediates the abnormal osteogenic and adipogenic capabilities of bone marrow mesenchymal stem cells from chronic graft-versus-host disease patients

Fig. 6

A The levels of IL-6, IL-10, IL-17, TNFa, IFN-r, and TGF-b in bone marrow serum from no cGVHD and active cGVHD patients. B Schematic diagram showing (i) the MSC co-cultrued with MLR conditioned medium collection process and (ii) CM_MSC cultrued with MLR conditioned medium collection process. C The levels of IL-6, IL-10, IL-17, TNFa, IFN-r, and TGF-b in the conditioned medium of cell-cell cocultured and MSCs cultured alone. D The immunosuppressive function of different concentrations of cocultured MSCs from HD, no cGVHD, and active cGVHD patients. E The paracrine immunosuppressive function of MSCs conditioned medium with MLR. F Representative protein expression of NF-kB, p-NF-kB, and GAPDH in MSCs derived from healthy donors, patients without cGVHD and patients with active cGVHD. HD-MSCs (n = 3), no cGVHD-MSCs (n = 6), and active cGVHD-MSCs (n = 12). Black bars in each figure represent the median ± range. NS, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

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