Fig. 3: Functional interaction between p38α and β-catenin in in vitro models of CRC. | Cell Death & Disease

Fig. 3: Functional interaction between p38α and β-catenin in in vitro models of CRC.

From: Pharmacological targeting of the novel β-catenin chromatin-associated kinase p38α in colorectal cancer stem cell tumorspheres and organoids

Fig. 3

A In vitro binding assay between GST-p38α fusion protein and HIS-β-catenin. Bound proteins were analyzed by immunoblotting using anti-GST and anti-HIS antibodies. B Co-immunoprecipitation with anti-HA and anti-FLAG antibodies in HEK293 cells overexpressing HA-p38α or FLAG-β-catenin. C Co-immunoprecipitation of endogenous p38α and β-catenin in the indicated cells. D and E Co-immunoprecipitation of endogenous p38α and β-catenin in nuclear and cytoplasmic fractions of the indicated cells. F Co-immunoprecipitation of endogenous p38α and β-catenin in nuclear and cytoplasmic fractions from C57BL/6 mice normal colon tissue and AOM-treated APCMin/+ mice adenocarcinoma tissue. Input corresponds to 10% of the lysate. Anti-IgGs were used as negative controls. Lamin B1: nuclear loading control; PDI: cytoplasmic loading control; N = nucleus, C = cytoplasm, β-cat = β-catenin.

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