Fig. 2: S34F mutant U2AF1 increases apoptosis and inhibits the viability of both K562 and SKM-1 cell lines. | Cell Death & Disease

Fig. 2: S34F mutant U2AF1 increases apoptosis and inhibits the viability of both K562 and SKM-1 cell lines.

From: U2AF1 mutation promotes tumorigenicity through facilitating autophagy flux mediated by FOXO3a activation in myelodysplastic syndromes

Fig. 2

A Transfection efficiency under the fluorescence microscope. Scale bar, 50 µm. Western blotting analysis of FLAG-tagged wild-type or mutated protein level in stably transfected cells. Western blotting analysis to determine the protein expression levels of U2AF1WT or U2AF1S34F in stably transfected cells. B CCK-8 assays for investigating the proliferation capacity of WT and S34F mutant U2AF1. C Annexin V-APC/7-AAD double staining was utilized to detect cellular apoptosis by flow cytometry in both K562 and SKM-1 cell lines after wild-type and S34F mutant U2AF1 treatment for 96 h. D Colony formation ability of wild-type and S34F mutant U2AF1 in the both is shown; right histogram represents quantification analysis. Scale bar, 100 µm. E Hoechst 33258 staining of the cellular nuclei was performed to obverse apoptosis in both K562 and SKM-1 cells. Scale bar, 40 µm. The data are presented as the mean ± SD as well as the representative of no less than two single experimental processes. *p < 0.05, **p < 0.01, and ***p < 0.001. WT wild-type, NC negative controls.

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