Fig. 4: S34F mutant U2AF1 induces G2/M cell cycle arrest mediated by FOXO3a activation. | Cell Death & Disease

Fig. 4: S34F mutant U2AF1 induces G2/M cell cycle arrest mediated by FOXO3a activation.

From: U2AF1 mutation promotes tumorigenicity through facilitating autophagy flux mediated by FOXO3a activation in myelodysplastic syndromes

Fig. 4

A ChIP-seq for FOXO3a binding to the promoter regions. Heatmaps and average intensity curves of ChIP assay reads for FOXO3a. B The representative micrographs and quantification of BrdU-incorporating cells (red) in both K562 and SKM-1 cell lines are shown. Nuclei counterstained with DAPI. Scale bar, 50 µm. C The representative flow cytometry analysis of the cell cycle distribution after efficient transfection for 96 h in both K562 and SKM-1 cells. S34F mutant U2AF1 induced the increase in the proportion of S-phase cells and significantly reduced the percentage of cells at G2/M phase. D The knockout efficiency for FOXO3a was determined by western blotting assays in SKM-1 cells. E Gene silencing of FOXO3a rescued the G2/M blocks induced by S34F mutant U2AF1 in SKM-1 cells. The data represent all experiments in triplicate for each cell line. *p < 0.05, **p < 0.01, and ***p < 0.001. WT wild-type, NC negative controls.

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