Fig. 3: SGLT2 glucose entry is essential to GC7-treated cell metabolism and survival.
From: Inhibition of eIF5A hypusination reprogrammes metabolism and glucose handling in mouse kidney
a, b Confluent PCT cells were treated or not with 30 µM GC7 for 24 h and then glycolysis in response to glucose addition (10 mM) was evaluated using Seahorse technology. Canagliflozin (10 µM) was added 4 h before analysis or along analysis as indicated. Dots displayed mean ± SD of three independent experiments. c Mortality evaluation using live/dead cells fluorescence assay of PCT cells co-treated with 10 µM canagliflozin and 30 µM GC7. Representative pictures for each group are displayed and show live (green, calcein-FITC) and dead (red, ethidium bromide homodimer) cells. Scale bar is indicated. Graphic displayed scatter plot of four independent values and mean ± SD. *p < 0.05 evaluated using Kruskal–Wallis (a, c) or Mann–Whitney (b) tests.
