Fig. 4: KIF18B promotes DU145 and PC-3 cell proliferation.
From: Kinesin family member 18B regulates the proliferation and invasion of human prostate cancer cells
A, B RT-qPCR analysis of KIF18B mRNA levels in A control and stable KIF18B-overexpressing DU145 and PC-3 cells and B DU145 and PC-3 cells transfected with shCtrl, shKIF18B#1, or shKIF18B#2. C, D Western blot analysis of KIF18B protein levels in C KIF18B-overexpressing DU145 and PC-3 cells and D DU145 and PC-3 cells transfected with shCtrl, shKIF18B#1, or shKIF18B#2. E, F CCK-8 cell viability assays of E KIF18B-overexpressing DU145 and PC-3 cells and F DU145 and PC-3 cells transfected with shCtrl, shKIF18B#1, or shKIF18B#2. G, H Colony formation assays of G KIF18B-overexpressing DU145 and PC-3 cells and H DU145 and PC-3 cells transfected with shCtrl, shKIF18B#1, or shKIF18B#2. Data are presented as the mean ± SD of at least three independent experiments and were analyzed with Student’s t test or one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001).
