Fig. 6: KIF18B activates the PI3K–AKT–mTOR signaling pathway.
From: Kinesin family member 18B regulates the proliferation and invasion of human prostate cancer cells
A PPI network of hub genes identified among the DEGs. (B) Heatmap of the expression pattern of hub genes. (C) and (D) Enrichment of DEGs in the PI3K–AKT–mTOR signaling pathway determined using KEGG, WikiPathways, PID, and Reactome databases. (E and F) Western blot analysis of PI3K–AKT–mTOR signaling pathway components in (E) KIF18B-overexpressing DU145 and PC-3 cells and (F) DU145 and PC-3 cells transfected with shCtrl, shKIF18B#1, or shKIF18B#2. Band intensities of total proteins were normalized to that of β-actin, and band intensities of phosphorylated proteins were normalized to that of the corresponding total proteins.
