Fig. 3: PEDF inhibits proliferation, migration, tube formation, and promotes apoptosis of lymphatic endothelial cells.
From: PEDF inhibits lymphatic metastasis of nasopharyngeal carcinoma as a new lymphangiogenesis inhibitor
A, B The proliferation of hLECs was examined by CCK8 assay (A) and EdU (B) after BSA or rPEDF treatment for 48 h (image magnification, 100×). The histogram represents the rate of proliferative hLECs. C The apoptotic hLECs were quantified by flow cytometry after incubation with BSA or 640 nM rPEDF for 48 h. The histogram represents the rate of apoptotic hLECs. D, E The migration of hLECs was measured with the cell scratch test (D) and transwell migration assay (E) after BSA or 640 nM rPEDF treatment for 12 h (image magnification, 200×). The histogram represents migrated cells per field. F Tube formation assay was used to test the lymphangiogenesis capacity in hLECs after BSA or 640 nM rPEDF treatment for 12 h (image magnification, 100×). The histogram represents the branch number per field. Scale bars: 100 μm (B, E, F), *p < 0.05, ***p < 0.001, the results are presented as mean ± SD.
