Fig. 5: PEDF inhibits the expression and secretion of VEGF-C in nasopharyngeal carcinoma cells.
From: PEDF inhibits lymphatic metastasis of nasopharyngeal carcinoma as a new lymphangiogenesis inhibitor
A, B The VEGF-C concentration of (A) S18 and (B) 5–8 F cells in the cell culture medium was detected by ELISA after BSA and 640 nM recombinant PEDF treatment for 48 h. C VEGF-C expression in S18, S26, 5–8 F, and 6–10B cells treated with different doses of rPEDF for 48 h, was detected by western blot. D VEGF-C expression in S18 and 5–8 F cells treated with 640 nM rPEDF for 0, 3, 6, 12, 24, and 48 h was detected by western blot. E S18 and 5–8 F cells were transfected with PEDF plasmid or vehicle plasmid for 48 h. PEDF and VEGF-C protein levels were measured by western blot. F Representative images of Matrigel tube formation assay in hLECs were cultured with conditioned medium derived from control NPC cells and PEDF overexpressing NPC cells (image magnification, 100×). Scale bars:100 μm (F), *p < 0.05, **p < 0.01, the results are presented as mean ± SD.
