Fig. 1: Wild-type SEC23B localizes to the nucleus.
From: Non-canonical role of wild-type SEC23B in the cellular stress response pathway
A Non-malignant thyroid Nthy-ori 3-1 cells were stably transduced with EGFP, wild-type SEC23B, and mutant p.Val594Gly SEC23B (the latter two fused with EGFP). Nthy-SEC23B-WT and Nthy-SEC23B-V594G cells show nuclear expression of SEC23B. Nthy-EGFP cell line is used as a control to show non-specific GFP subcellular localization. Blue, DAPI; green, SEC23B-EGFP or EGFP. Scale bars, 25 μm. B Protein levels after nuclear–cytoplasmic fractionation in stably transduced Nthy-ori 3-1 cells detects endogenous and EGFP-tagged SEC23B in the nuclear compartment. Nthy-EGFP cell line is used as a control to indicate baseline endogenous levels of SEC23B. PARP1, nuclear marker; GAPDH, cytoplasmic marker; CANX, endoplasmic reticulum marker. C Protein levels after nuclear–cytoplasmic fractionation in parental HEK293 cells detects endogenous SEC23B in the nuclear compartment. PARP1 and Lamin A/C, nuclear markers; GAPDH, cytoplasmic marker; CANX, endoplasmic reticulum marker.
