Fig. 4: Global overview of the SEC23B interactome.
From: Non-canonical role of wild-type SEC23B in the cellular stress response pathway
A Immunofluorescence staining of stably transduced Nthy-ori 3-1 thyroid cells for aggresomes. Blue, DAPI; green, SEC23B-EGFP or EGFP. Scale bars, 25 µm. B Experimental design of the mass spectrometry experiment with and without MG132 treatment. IgG immunoglobulin, IP immunoprecipitation. C Global number of interacting partners in Nthy-SEC23B-WT and Nthy-SEC23B-V594G cells with and without MG132 treatment. Only protein interactants with spectral count ratios ≥2 relative to the IgG and Nthy-EGFP immunoprecipitations (negative controls) are depicted. D Overlapping and unique interacting partners between untreated and MG132-treated Nthy-SEC23B-WT and Nthy-SEC23B-V594G cells. Only proteins with spectral counts ≥10 are included in this Venn diagram. Table S2 includes the complete list of interacting proteins. E Heatmaps of the top 35 interacting proteins based on spectral counts in wild-type Nthy-SEC23B-WT and mutant Nthy-SEC23B-V594G cells. COPII component and related proteins (SEC23B, SEC23A, SEC24A-D, SEC31A, SEC23IP, SEC16A) serve as positive controls. Table S2 includes the complete list of interacting proteins. Sqrt square root. F Subcellular localization analysis using Ingenuity Pathway Analysis (IPA) indicates that a subset (16–23%) of the SEC23B-interacting proteins is predicted to reside in the nucleus. G Pathway analysis of SEC23B-interacting proteins predicted to reside in the nucleus.
