Fig. 6: TCLlnc1 physically interacted with heterogeneous nuclear ribonucleoprotein D (HNRNPD) and Y-box binding protein-1 (YBX1). | Cell Death & Disease

Fig. 6: TCLlnc1 physically interacted with heterogeneous nuclear ribonucleoprotein D (HNRNPD) and Y-box binding protein-1 (YBX1).

From: A novel lncRNA TCLlnc1 promotes peripheral T cell lymphoma progression through acting as a modular scaffold of HNRNPD and YBX1 complexes

Fig. 6

A Nuclear location of TCLlnc1 in Jurkat cells (red). Nuclei were stained by 4′, 6-diamidino-2-phenylindole (DAPI, blue). B TCLlnc1-binding proteins detected by mass spectrometry. C Western blot validation of proteins pulled down with TCLlnc1. D Interaction of TCLlnc1 with HNRNPD and YBX1 detected by RNA immunoprecipitation (RIP) assay. The fold enrichment of TCLlnc1 in RIP is relative to its matching IgG control RIP. LncRNA linc00152 was referred as a negative control. E Expression of TCLlnc1, HNRNPD, and YBX1 after indicated RNA knockdown detected by qRT-PCR and western blot. F Western blot analysis of HNRNPD and YBX1 pulled down by full-length or truncated TCLlnc1. G Interaction of HNRNPD and YBX1 after TCLlnc1 knockdown detected by co-immunoprecipitation (co-IP).

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