Fig. 2: Characteristics of circ‐HMGA2 in LUAD cells. | Cell Death & Disease

Fig. 2: Characteristics of circ‐HMGA2 in LUAD cells.

From: Circ-HMGA2 (hsa_circ_0027446) promotes the metastasis and epithelial-mesenchymal transition of lung adenocarcinoma cells through the miR-1236-3p/ZEB1 axis

Fig. 2

A The schematic illustration showing that circ-HMGA2 is cyclized from exons 2-3 of HMGA2. B Sanger sequencing showed the back-splice junction sites in circ-HMGA2. C Gel electrophoresis was performed to analyze RNase R digestion. Circ‐HMGA2 was resistant to RNase R digestion. D Upon exposure to actinomycin D, circ-HMGA2 was more stable than its linear form. E The relative expression levels of circ-HMGA2 in LUAD tissues and adjacent normal tissues were determined by qRT-PCR. F Circ-HMGA2 was significantly highly expressed in LUAD cells. G Circ-HMGA2 was localized mainly in the cytoplasm of H1299 cells, as verified by FISH. Scale bar: 25 μm. *P < 0.05.

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