Fig. 5: Circ-HMGA2 acts as a sponge for miR-1236-3p. | Cell Death & Disease

Fig. 5: Circ-HMGA2 acts as a sponge for miR-1236-3p.

From: Circ-HMGA2 (hsa_circ_0027446) promotes the metastasis and epithelial-mesenchymal transition of lung adenocarcinoma cells through the miR-1236-3p/ZEB1 axis

Fig. 5

A Analysis of the miRanda database indicated that circ-HMGA2 contains a binding site for miR-1236-3p. B The relative expression levels of miR-1236-3p in LUAD tissues and adjacent normal tissues were determined by qRT-PCR. C Transwell assays were performed in H1299 and A549 cells after transfection of the miR-1236-3p inhibitor. Scale bar: 200 μm. D miR-1236-3p inhibited EMT progression in LUAD cells. E Schematic illustration of circ-HMGA2 luciferase reporter vectors containing the wild-type (WT) or mutant (Mut) putative miR-1236-3p binding sites. F Luciferase activity was analyzed in 293T cells after co-transfection with miR-1236-3p mimics, miR-NC or miR-1236-3p inhibitors, and the WT or Mut luciferase reporter vectors. The relative luciferase activity was presented by the relative hRluc/hluc ratio. G The FISH results showed that circ-HMGA2 and miR-1236-3p are co-localized in the cytoplasm. Scale bar: 8 μm. *P < 0.05, **P < 0.01.

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