Fig. 1: Verification of EndMT in a mouse model of pulmonary fibrosis. | Cell Death & Disease

Fig. 1: Verification of EndMT in a mouse model of pulmonary fibrosis.

From: Trajectory modeling of endothelial-to-mesenchymal transition reveals galectin-3 as a mediator in pulmonary fibrosis

Fig. 1

A Schematic diagram of the construction of the IPF model in Tie2creER/+;Rosa26tdTomato/+ mice based on a C57bl/6 genetic background. B Immunofluorescence imaging of ECs (CD31, red) and fibroblasts (COL1, green) in the BLM/control groups. The region in blue represents nucleus. Asterisks indicate the co-localization of ECs and fibroblasts. Scale bar = 13 μm. C Comparative analysis of the relative proportions of EC-derived cells (Tie2+) and ECs (Tie2+CD31+) in the BLM/control groups presented as means ± SD, n = 2. x-axis: different mice derived from C57BL/6. Tie2-positive cells represent all cells of endothelial origin; Tie2 + CD31 + cells represent resident ECs that remain endothelium-feature supplemented with BLM or saline solution. y-axis: the percentage of subpopulations of Tie2-positive and Tie2CD31-positive cells. n = 2. D Gating strategy for calculating the fraction that underwent EndMT (Tie2+Vim+) cultured in vitro for 72 h after isolation from lungs by PBS perfusion. Specifically, cells gated by rectangles in black are subpopulation of interest.

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