Fig. 2: Transcriptional heterogeneity of ECs during EndMT in IPF at single-cell level. | Cell Death & Disease

Fig. 2: Transcriptional heterogeneity of ECs during EndMT in IPF at single-cell level.

From: Trajectory modeling of endothelial-to-mesenchymal transition reveals galectin-3 as a mediator in pulmonary fibrosis

Fig. 2

A Sing-cell transcriptome sequencing (scRNA-seq) using the Fluidigm C1 platform. Lung cells from the BLM and control groups were collected and sorted by FACS to harvest Tie2+ cells for scRNA-seq. B UMAP visualization of single-cell transcriptomes in the BLM and control groups. Left panel: colored by origin of cells (BLM or control). Right panel: colored according to the unsupervised clustering of cells. C Cluster-wide expression of EndMT-related markers/regulators screened among differentially expressed genes of high significance (p < 0.01) based on their GO-terms. D Expression levels of candidate regulators in whole lung samples from the BLM (day 7) and control groups according to RT-PCR. The data are presented as means ± SD, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001.

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