Fig. 7: Oleandrin induces ICD via ER stress. | Cell Death & Disease

Fig. 7: Oleandrin induces ICD via ER stress.

From: Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2α/ATF4/CHOP pathway in breast cancer

Fig. 7

MCF7, T47D, and MDA-MB-231 cells were treated with oleandrin or DMSO for 10 h. The differential of mRNA was analyzed by mRNA sequencing. A The Venn’s diagrams show the significant difference mRNA in three different pairs of cells. B The pathway analysis was performed functional enrichment of these different mRNA in GO and KEGG databases. C The heatmap was generated based on the significant different genes in ATF2 pathway. D, E The ATF3, ATF4, and CHOP (DDIT3) expression was detected by qRT-PCR and western blotting. Data are presented as mean ± standard error of the mean from three independent experiments. **p < 0.01. ole, oleandrin.

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