Fig. 5: LINC00470 activated ZNF131 to inhibit the transcription of APEX1. | Cell Death & Disease

Fig. 5: LINC00470 activated ZNF131 to inhibit the transcription of APEX1.

From: LINC00470 accelerates the proliferation and metastasis of melanoma through promoting APEX1 expression

Fig. 5

A The interaction of LINC00470 and ZNF131 was detected by RIP assay in normal and melanoma tissues. B, C RIP assay showed the enrichment of LINC00470 and ZNF131 in melanoma cells or the AR-overexpressed melanoma cells. D RNA pull-down and western blot analysis after the cells were transfected with OE-NC and OE-LINC004705. E CHIP assay analysis after the cells were transfected with control, OE-NC, OE-LINC00470, and OE-LINC00470 with OE-ZNF131. F Dual-luciferase assay was used to investigate the interaction between ZNF131 and APEX1. G, H qRT-PCR and WB assay showed the expression level of APEX1 in ZNF131-overexpressed melanoma cells. qRT-PCR analysis of ZNF131 (I) and APEX1 (J) in melanoma tissues and normal tissues. K The protein expression of APEX1 and ZNF131 in melanoma and normal tissues using western blot. IHC assay was conducted to evaluate the levels of ZNF131 (L) and APEX1 (M) in melanoma and normal tissues. The mean ± SD in the graph shows the relative levels from three replicates. *p < 0.05, **p < 0.01.

Back to article page