Fig. 2: Exogenous overexpression of Runx2 enhances the proliferation and migration of ccRCC cells. | Cell Death & Disease

Fig. 2: Exogenous overexpression of Runx2 enhances the proliferation and migration of ccRCC cells.

From: A Zic2/Runx2/NOLC1 signaling axis mediates tumor growth and metastasis in clear cell renal cell carcinoma

Fig. 2

A The expression level of Runx2 in one human immortalized renal epithelial cell and five ccRCC cells was analyzed by qRT-PCR and western blot analyses, respectively. β-Actin was also tested as a loading control. B Plasmid-mediated transfection was used to overexpress the Runx2 in CAKI-1 and SKRC39 cells, and the expression level of Runx2 was confirmed by qRT-PCR and western blot analyses. C Foci formation assay showed that overexpression of Runx2 promoted ccRCC cells growth. D Migration assay suggested that the migration ability of ccRCC cells was increased after overexpression of Runx2, compared to control cells. In all panels, *, P < 0.05; **, P < 0.01.

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