Fig. 5: Zic2 upregulates the expression of Runx2 promoting ccRCC cell growth and metastasis. | Cell Death & Disease

Fig. 5: Zic2 upregulates the expression of Runx2 promoting ccRCC cell growth and metastasis.

From: A Zic2/Runx2/NOLC1 signaling axis mediates tumor growth and metastasis in clear cell renal cell carcinoma

Fig. 5

A IHC staining analysis in ccRCC tissue array showed the positive correlation of Zic2 and Runx2 expressions. B Western blot analysis showed the downregulation of Runx2 in 786-O and ACHN cells after knockdown of Zic2. β-Actin was also tested as a loading control. C qRT-PCR was used to analyze the expression level of Zic2 in ccRCC and paired normal renal tissues (n = 29). D Survival curves based on IHC staining indicated the poorer outcome of ccRCC patients with high expression of Zic2 than those with low level of Zic2 (P < 0.0001). E–G MTT test (E), EdU incorporation (F), and foci formation (G) assays were performed to analyze the proliferation of 786-O and ACHN cells after knockdown of Zic2 or/and Runx2 overexpression, respectively. H The migration ability of ccRCC cells after interference of Zic2 or/and overexpression of Runx2 was tested by Transwell migration assay. In all panels, *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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