Fig. 1: The expression of m6A demethylase FTO is decreased by HIRI. | Cell Death & Disease

Fig. 1: The expression of m6A demethylase FTO is decreased by HIRI.

From: N6-methyladenosine demethylase FTO impairs hepatic ischemia–reperfusion injury via inhibiting Drp1-mediated mitochondrial fragmentation

Fig. 1

A Representative histopathologic images of liver sections harvested at 24 h after reperfusion (H&E staining; scale bars, 100 μm). The damage degree was graded using Suzuki criteria. B Blood samples were collected to determine the ALT and AST levels (U/L). C The total level of methylated RNA (m6A) in hepatic tissues was detected by EpiQuik m6A RNA Methylation Quantification Kit. D The total level of methylated RNA (m6A) in hepatic tissues was detected by dot blotting analysis. E The expressions of major m6A methyltransferases (METTL3, METTL14, RBM15, WTAP, and VIRMA) and demethylases (FTO and ALKBH5) at the mRNA level were examined by qRT-PCR. F The expression of FTO at the mRNA level was examined by qRT-PCR. G The expression of FTO at the protein level was verified by Western blotting analysis. H The expression of FTO at the protein level was verified by IHC. N = 5; *P < 0.05 and **P < 0.01, ***P < 0.001 vs. sham group.

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