Fig. 5: Liver-specific overexpression of FTO suppresses the IRI-elevated expression of Drp1 in hepatocytes upon IRI in vivo and in vitro. | Cell Death & Disease

Fig. 5: Liver-specific overexpression of FTO suppresses the IRI-elevated expression of Drp1 in hepatocytes upon IRI in vivo and in vitro.

From: N6-methyladenosine demethylase FTO impairs hepatic ischemia–reperfusion injury via inhibiting Drp1-mediated mitochondrial fragmentation

Fig. 5

A Me-RIP qRT-PCR analysis was performed to evaluate the methylated Drp1 RNA level in the hepatocytes and hepatic tissues infected with control AAV8-TBG-null and AAV8-TBG-FTO during IRI. B qRT-PCR was adopted to analyze the expression of Drp1 at the mRNA level in the hepatocytes and hepatic tissues infected with control AAV8-TBG-null and AAV8-TBG-FTO during IRI. C Western blotting analysis was used to verify the expressions of total and activated Drp1 (pSer616) at the protein level in the hepatocytes and hepatic tissues infected with control AAV8-TBG-null and AAV8-TBG-FTO during IRI. N = 3; *P < 0.05 and **P < 0.01 vs. AAV8-TBG-NC group.

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