Fig. 6: Drp1 is a downstream target of FTO in the progression of HIRI. | Cell Death & Disease

Fig. 6: Drp1 is a downstream target of FTO in the progression of HIRI.

From: N6-methyladenosine demethylase FTO impairs hepatic ischemia–reperfusion injury via inhibiting Drp1-mediated mitochondrial fragmentation

Fig. 6

A qRT-PCR analysis showed that overexpression of wild-type but not demethylase- mutated FTO decreased the Drp1 mRNA level in isolated hepatocytes. B Western blotting analysis showed that overexpression of wild-type but not demethylase-mutated FTO decreased the Drp1 protein level in isolated hepatocytes. C Me-RIP qRT-PCR analysis showed that overexpression of wild-type but not demethylase-mutated FTO decreased the methylated Drp1 level in isolated hepatocytes. D The curve and statistical analysis of Drp1 mRNA decay slope on the transfection of FTO siRNA, FTO-WT, FTO-Mut (R96Q), or the negative control after transcriptional inhibition (TI) were shown. N = 3; *P < 0.05 versus indicated group.

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