Fig. 2: ADCK1 promoted the anchorage-independent growth and invasion of colon cancer cells. | Cell Death & Disease

Fig. 2: ADCK1 promoted the anchorage-independent growth and invasion of colon cancer cells.

From: ADCK1 activates the β-catenin/TCF signaling pathway to promote the growth and migration of colon cancer cells

Fig. 2

A Overexpression of ADCK1 in SW620 and RKO cells. Cells were infected with lentivirus, and the expression of exogenous ADCK1 (Flag-ADCK1) was examined using western blotting. B An EdU assay was performed to examine the roles of ADCK1 in cell proliferation. The details are described in the “Experimental procedures” section. Percentage of positively stained cells= number of positively stained cells/number of total cells. C The effects of ADCK1 overexpression on the anchorage-independent growth of SW620 and RKO cells were evaluated using a soft agar assay. The details are described in the “Experimental procedures” section. The colonies were counted, and statistical analysis was performed. D, E The effects of ADCK1 overexpression on the invasion of RKO cells were evaluated using a Transwell assay. **P < 0.01. The scale bars were indicated.

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