Fig. 4: Knockdown of ADCK1 inhibited tumorigenesis.

A The effects of ADCK1 knockdown on organoid formation were assessed. The tumors of C57BL/6J-Apc^Min/J mice were used for organoid culture as described in the “Experimental procedures” section. The number of organoids (>100 µm) was quantified. B, C The effects of ADCK1 knockdown on tumor formation by SW620 cells were assessed. SW620 cells with ADCK1 knockdown and control SW620 cells were subcutaneously injected into nude mice. The tumors were harvested, the tumor volume and weight were measured, and IHC was performed to examine the expression of ADCK1. D, E The effects of ADCK1 knockdown on the distant seeding of SW620 cells were evaluated in nude mice as described in the “Experimental procedures” section. The lungs of the mice were collected, and the metastatic foci were examined using HE staining. The scale bars were indicated. **P < 0.01.