Fig. 2: hsa_circ_101555 localizes in cytoplasm. | Cell Death & Disease

Fig. 2: hsa_circ_101555 localizes in cytoplasm.

From: Circular RNA hsa_circ_101555 promotes hepatocellular carcinoma cell proliferation and migration by sponging miR-145-5p and regulating CDCA3 expression

Fig. 2

A hsa_circ_101555 in HCC tumor and adjacent nontumor tissues detected by FISH. B Expression of hsa_circ_101555 in ten HCC tumor and adjacent normal tissues measured by qRT-PCR (***p < 0.001). C Expression of hsa_circ_101555 in multiple HCC cell lines (*P < 0.05; **P < 0.01; n = 4). D Linear 101555 is readily digested by RNase R, while hsa_circ_101555 is not (***P < 0.001; n = 4). E, F FISH confirmed that hsa_circ_101555 is predominantly located in cytoplasm. Nuclei stained with DAPI. U6, 18 S, and hsa_circ_101555 labeled with cy3 (n = 4). G Diagrammatic representation of siRNA targeting the back-splice junction (si-hsa_circ_101555-#1, si-hsa_circ_101555-#2, and si-hsa_circ_101555-#3); qRT-PCR results for hsa_circ_101555 expression in HCCLM3 and HepG2 cells treated, with or without siRNA. si-NC, control oligonucleotides with scramble sequence; si-hsa_circ_101555-#1, si-hsa_circ_101555-#2, and si-hsa_circ_101555-#3, oligonucleotides targeting the back-splice junction (***P < 0.001; n = 4). H qRT-PCR results for hsa_circ_101555 in Huh-7 and SK-Hep-1 cells treated with lv-NC or lv- hsa_circ_101555 (**P < 0.01; n = 4).

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