Fig. 8: hsa_circ_101555 promotes HCC tumor growth in vivo. | Cell Death & Disease

Fig. 8: hsa_circ_101555 promotes HCC tumor growth in vivo.

From: Circular RNA hsa_circ_101555 promotes hepatocellular carcinoma cell proliferation and migration by sponging miR-145-5p and regulating CDCA3 expression

Fig. 8

A Schematic diagram shows that HCCLM3 cells were injected subcutaneously into BALB/c nude mice for 15 days to create tumor xenografts. After 15 days, 10 nmol cholesterol-modified hsa_circ_101555 shRNA or negative control shRNA was subcutaneously injected at the tumor site every 3 days for 18 days. B Representative images of HCC tumor bearing BALB/c nude mice. C Body weight of xenograft nude mice. D Body weight measured every 3 days. E Tumor volumes measured every 3 days (*P < 0.05; **P < 0.01; ***P < 0.001; n = 4). F Relative weights of tumors (***P < 0.001; n = 4). G IHC staining and IHC analysis of Ki-67 expression in subcutaneous xenograft tumors. Knockdown of hsa_circ_101555 downregulates Ki-67 expression (*P < 0.05; n = 4). H–J Expression of hsa_circ_101555, miR-145-5p and CDCA3 in subcutaneous xenograft tumors (**P < 0.01; ***P < 0.001; n = 4). K Protein expression level of CDCA3 in seven paired HCC and normal tissues (**P < 0.01; n = 4). L IHC staining and IHC analysis of CDCA3 expression in subcutaneous xenograft tumors. Knockdown of hsa_circ_101555 downregulates CDCA3 expression (**P < 0.01; n = 4). M Schematic diagram shows that hsa_circ_101555 promotes HCC cells proliferation, migration, and invasion through the miR-145-5p/CDCA3 axis.

Back to article page