Fig. 5: TFEB is a downstream target of Sox2, Oct4, and Nanog. | Cell Death & Disease

Fig. 5: TFEB is a downstream target of Sox2, Oct4, and Nanog.

From: TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy–lysosomal biogenesis

Fig. 5

a Putative binding sites of Oct3/4, Sox2, and Nanog at mouse TFEB promoter site. b Overexpression of Sox2, Oct4, and Nanog increases TFEB promoter-driven luciferase activity in HEK293T cells. c, d Knockdown of Sox2, Oct3/4, and Nanog in undifferentiated mESCs reduces endogenous TFEB mRNA (c) and TFEB protein levels (d). e–g Mutation in putative Sox2, Oct4, or Nanog binding sites (Sox2 MT/Oct4 MT/Nanog MT) in TFEB promoter reduces the TFEB promoter-driven luciferase activity in mESCs. Luciferase activity was evaluated 24 h after transfection. h–j Endogenous binding of the promoter sequences for Sox2, Oct4, or Nanog at the putative binding sites in TFEB promoter in mESCs was evaluated through ChIP-qPCR analysis. mRNA was normalized with β-actin. k Schematic diagram of TFEB in the regulation of pluripotency transcriptional network (PTN) in undifferentiated mESCs. All statistical analyses represent average values of a representative experiment from at least two independent experiments. Error bars represent SD values of triplicate assays. Data are shown as mean ± SD, n = 3. *p < 0.05; **p < 0.01; ***p < 0.001 ****p < 0.0001 compared to the corresponding control group. The student’s t test was used for all statistical analysis.

Back to article page