Fig. 8: HCQ enhanced the antitumor effect of FX11 in vitro and in vivo. | Cell Death & Disease

Fig. 8: HCQ enhanced the antitumor effect of FX11 in vitro and in vivo.

From: LDHA induces EMT gene transcription and regulates autophagy to promote the metastasis and tumorigenesis of papillary thyroid carcinoma

Fig. 8

A B-CPAP cells with stable LDHA knockdown were treated with the autophagy inhibitor hydroxychloroquine (HCQ). Western blotting was used to detect p62 and LC3BII/I levels. B Apoptosis of B-CPAP cells exposed to 10 μM of FX11 for 24 h with/without HCQ (10 μM for 24 h) was detected by flow cytometry. The data are expressed as the mean ± SD. C B-CPAP cells were exposed to FX11 (10 μM for 24 h) with/without HCQ (10 μM for 24 h), and western blotting was used to detect cleaved caspase 3 levels. D FX11 (3 mg/kg) with/without HCQ (60 mg/kg) was given to mice bearing papillary thyroid tumors for 21 days. Tumor weights were measured on day 30 E, and tumor volumes F are presented with growth curves. The data are presented as the mean ± SD. G H&E staining and representative IHC images of cleaved caspase 3 expression and Ki-67 staining of xenografted tumor specimens from the FX11 with/without HCQ groups are shown. All *p < 0.05, **p < 0.01, ***p < 0.001.

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