Fig. 2: HOTAIR regulates cell autophagy. | Cell Death & Disease

Fig. 2: HOTAIR regulates cell autophagy.

From: LncRNA-HOTAIR activates autophagy and promotes the imatinib resistance of gastrointestinal stromal tumor cells through a mechanism involving the miR-130a/ATG2B pathway

Fig. 2

A, B Western blotting was performed to detect the levels of autophagy-related proteins in GIST cells after transfection. C, D Confocal microscopy: red dots indicate autolysosomes. #P < 0.05, ##P < 0.01 vs. imatinib: yellow dots indicate autophagosomes. *P < 0.05, **P < 0.01 vs. imatinib. E Autophagosomes were observed by electron microscopy. F CCK-8 assays were performed after GIST cells were treated in four different ways (negative control, 3-MA, pEX-3-HOTAIR, and 3-MA plus pEX-3-HOTAIR). **P < 0.01, ***P < 0.001 vs. control or pEX-3-HOTAIR. G CCK-8 assays were performed after GIST cells were treated in four different ways (negative control, RAPA, siHOTAIR, RAPA plus siHOTAIR). *P < 0.05, **P < 0.01, ***P < 0.001 vs. control or siHOTAIR. H Flow cytometry analysis to determine the effect of HOTAIR in rescuing GIST cells from apoptosis. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control or Imatinib + pEX-3-HOTAIR.

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